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polA [2020-09-29 09:54:32]
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polA [2020-09-29 09:54:32]

DNA polymerase I, required for repair of UV- or MMS-induced DNA damage, processing of Okazaki fragments
locus
BSU_29090
pI
4.92
mw
98.89 kDa
protein length
880 aa Sequence Blast
gene length
2643 bp Sequence Blast
function
DNA replication
product
DNA polymerase I
essential
no
ec
2.7.7.7
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

    • Gene

    Coordinates
    2,973,182 2,975,824

    Phenotypes of a mutant

  • a [gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA] [gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|exoR] double mutant is not viable (synthetic lethality) [pubmed|17114254]
  • strongly reduced stationary phase mutagenesis [Pubmed|27399782]

    • The protein

    Catalyzed reaction/ biological activity

  • has DNA polymerase and 5'-3' exonuclease activity, but no 3'-5' exonuclease activity [Pubmed|16045613]
  • maturation of Okazaki fragments (together with [protein|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|RNase HIII]) [pubmed|30670546]
  • 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)

    • Protein family

  • DNA polymerase type-A family (single member, according to UniProt)

    • Paralogous protein(s)

  • [protein|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|ExoR]:

    • [SW|Domains]

  • 5'-3' exonuclease domain (aa 174-268) (according to UniProt)
  • 3'-5' exonuclease domain (aa 302-470) (according to UniProt)

    • Structure

  • [PDB|6VDE] (from Mycobacterium smegmatis, 40.5% identity) [pubmed|32034423]
  • [PDB|1XWL] (large fragment, corresponds to AA 310 to 880, Geobacillus stearothermophilus)

    • [SW|Localization]

  • recruited to replication forks after induction of DNA damage [pubmed|31251806]

    • Expression and Regulation

    Operons

    genes
    [gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA]-[gene|EC96EDED2472607DFA4C5EBA054157F7832EE41C|mutM]-[gene|3BEE8E248BC583FCCB37B7A209A122DD93CD398C|ytaF]-[gene|E163A282560DB882B81E783E7E95E1960F4AE4D2|ytaG]
    description
    [pubmed|22383849]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|23396918], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
    • view in new tab

    Biological materials

    Mutant

  • GP3532 (del spec), available in [SW|Jörg Stülke]'s lab
  • BKE29090 ([gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATTTCCTCCTAAGA, downstream forward: _UP4_TAAACAGAGATAGGAAGTGA
  • BKK29090 ([gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATTTCCTCCTAAGA, downstream forward: _UP4_TAAACAGAGATAGGAAGTGA

    • References

  • 17114254,16045613,17905985,27399782,30670546,30726292,31251806,32034423