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polA [2020-09-29 09:54:32]
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polA [2020-09-29 09:54:32]

DNA polymerase I, required for repair of UV- or MMS-induced DNA damage, processing of Okazaki fragments
Locus
BSU_29090
Isoelectric point
4.92
Molecular weight
98.89 kDa
Protein length
880 aa Sequence Blast
Gene length
2643 bp Sequence Blast
Function
DNA replication
Product
DNA polymerase I
Essential
no
E.C.
2.7.7.7
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Genetics → DNA replication
  • Information processing → Genetics → DNA repair/ recombination
  • Information processing → Genetics → DNA repair/ recombination → Other proteins

    • Gene

    Coordinates
    2,973,182 2,975,824

    Phenotypes of a mutant

  • a polA exoR double mutant is not viable (synthetic lethality) PubMed
  • strongly reduced stationary phase mutagenesis PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • has DNA polymerase and 5'-3' exonuclease activity, but no 3'-5' exonuclease activity PubMed
  • maturation of Okazaki fragments (together with RNase HIII) PubMed
  • 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)

    • Protein family

  • DNA polymerase type-A family (single member, according to UniProt)

    • Paralogous protein(s)

  • ExoR:

    • Domains

  • 5'-3' exonuclease domain (aa 174-268) (according to UniProt)
  • 3'-5' exonuclease domain (aa 302-470) (according to UniProt)

    • Structure

  • 6VDE (from Mycobacterium smegmatis, 40.5% identity) PubMed
  • 1XWL (large fragment, corresponds to AA 310 to 880, Geobacillus stearothermophilus)

    • Localization

  • recruited to replication forks after induction of DNA damage PubMed

    • Expression and Regulation

    Operons

    Genes
    polA-mutM-ytaF-ytaG
    Description
    PubMed

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon
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    Biological materials

    Mutant

  • GP3532 (del spec), available in Jörg Stülke's lab
  • BKE29090 (polA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCAATTTCCTCCTAAGA, downstream forward: _UP4_TAAACAGAGATAGGAAGTGA
  • BKK29090 (polA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCAATTTCCTCCTAAGA, downstream forward: _UP4_TAAACAGAGATAGGAAGTGA

    • References

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